Validating a rapid method for detecting common polymorphisms in the APOA5 gene by melting curve analysis using LightTyper.
نویسندگان
چکیده
Diagn 2000;20:976–8. 10. Shahabi S, Oz UA, Bahado-Singh RO, Mahoney MJ, Omrani A, Baumgarten A, et al. Serum hyperglycosylated hCG: a potential screening test for fetal Down syndrome. Prenat Diagn 1999;19:488–9. 11. Pandian R, Lu J, Ossolinska-Plewnia J. Fully automated chemiluminometric assay for hyperglycosylated human chorionic gonadotropin (invasive trophoblast antigen). Clin Chem 2003;49:808–10. 12. Central Bureau of Statistics. Voorburg/Heerlen, The Netherlands: CBS, 2004. 13. Cole LA, Omrani A, Cermik D, Bahado-Singh RO, Mahoney MJ. Hyperglycosylated hCG, a potential alternative to hCG in Down syndrome screening. Prenat Diagn 1998;18:926–33.
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عنوان ژورنال:
- Clinical chemistry
دوره 51 7 شماره
صفحات -
تاریخ انتشار 2005